Isotype control antibodies serve as negative controls that help distinguish specific antibody staining from non-specific background staining in experiments. They are essential for the accurate assessment of antibody drug actions and efficacy in both in vitro and in vivo monoclonal antibody (mAb) studies. Isotype controls are most commonly used in flow cytometry (FC), immunoprecipitation (IP), and immunohistochemistry (IHC), but can also be utilized in other applications such as Western blotting (WB), enzyme-linked immunosorbent assay (ELISA), and immunofluorescence/immunocytochemistry (IF/ICC) for blocking and coating purposes.
Q:How to Choose an Appropriate Homogeneous Control Group？
A:Select the same species, the same type of immunoglobulin, the same subclass of immunoglobulin (for certain species), and a tag that matches the primary antibody (such as biotin, HRP, fluorophores, etc.).
Genomeditech develops in vivo isotype controls, providing high-purity monoclonal antibodies with extremely low endotoxin levels, sterile conditions, no mycoplasma, and no MVM, ensuring a range of references and guarantees for your experiments.
