In transient transfection, the probability of integrating foreign genes into the genome is very low. The majority of the foreign genes remain in free plasmid form, and as the cells are passaged, the plasmid is gradually lost, leading to dilution of gene copies and preventing sustained expression of the foreign gene. Transfection is typically performed using reagents, and the expression is transient.
When foreign DNA is cloned into a lentiviral vector with a resistance marker and packaged into a virus, the lentivirus can infect target cells. After successful infection, the lentiviral genome integrates into the host cell's chromosome. The resistance marker in the vector allows for selection; cells that have been successfully infected will survive in the culture medium, while uninfected cells will not. Under the selective pressure of the resistance marker, stable cell lines that express the target protein or silence specific genes can be successfully generated.
With years of experience in cell culture and viral packaging, Genomeditech offers a cell line construction service. The service involves infecting target cells with lentivirus carrying foreign genes, and through screening, stable cell lines expressing the target gene can be obtained, which are ideal for gene function studies.
Genomeditech Cell Line Construction Platform
Overexpression Cell Lines
1.1 Mixed Clonal Cell Lines
1.2 Monoclonal Cell Lines
sh Knockdown Cell Lines
2.1 shRNA Custom Cell Lines
2.2 shRNA Cell Line Set
Gene Editing Cell Lines
3.1 Knockout Cell Lines / Conditional Knockout
3.2 Knock-in Cell Lines
3.3 Gene Transcription Activation / Inhibition Cell Lines
3.4 Point Mutation Cell Lines
3.5 Methylated / Demethylated Cell Lines
Reporter Gene Cell Lines
4.1 LUC Reporter Cell Lines
