Plasmid — The Most Common Vector in Genetic Engineering
A plasmid is a small DNA molecule that exists independently within a cell, separate from the cell's chromosomal or nucleoid DNA, and is capable of autonomous replication. It is also referred to as an episome or cytoplasmic plasmid. The vast majority of plasmids are closed circular double-stranded DNA molecules.
◆ Stable and easily amplified.
◆ Capable of transient expression for short-term function, but can also be used to create stable cell lines.
◆ A commonly engineered vector can accommodate relatively large target genes.
