A reporter gene is a gene that encodes a protein or enzyme that can be easily identified, and when fused with a target gene, its expression product can be used to monitor the regulation of the target gene's expression. Reporter genes are widely used in studies of gene regulation, RNA interactions, and related fields.
Genomeditech uses the Dual-Luciferase Reporter Assay System to provide reporter gene detection services. This system consists of firefly luciferase and Renilla luciferase, allowing specific transcriptional regulatory elements or binding site sequences to be cloned into a luciferase reporter vector. Changes in luciferase activity are then measured to reflect the transcriptional regulation of the gene of interest. This technique has been widely applied to studies investigating molecular binding mechanisms.