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Current position:Technical Service > Reporter Gene Detection Services
Reporter Gene Detection Services
Introduction

A reporter gene is a gene that encodes a protein or enzyme that can be easily identified, and when fused with a target gene, its expression product can be used to monitor the regulation of the target gene's expression. Reporter genes are widely used in studies of gene regulation, RNA interactions, and related fields.


Genomeditech uses the Dual-Luciferase Reporter Assay System to provide reporter gene detection services. This system consists of firefly luciferase and Renilla luciferase, allowing specific transcriptional regulatory elements or binding site sequences to be cloned into a luciferase reporter vector. Changes in luciferase activity are then measured to reflect the transcriptional regulation of the gene of interest. This technique has been widely applied to studies investigating molecular binding mechanisms.



双荧光素酶原理图.png

Features

The Dual-Luciferase Reporter Assay System is a dual-reporter system that uses luciferin and coelenterazine as substrates to detect the activity of firefly luciferase and Renilla luciferase, respectively. Firefly luciferase acts as the reporter gene, catalyzing the oxidation of luciferin into oxyluciferin, a process that emits bioluminescence. Renilla luciferase serves as the internal control, catalyzing the oxidation of coelenterazine into coelenteramide, which also emits bioluminescence.

Bioluminescence produced during the reaction can be measured using a luminometer or a microplate reader to detect changes in luciferase activity.


Key features of the Dual-Luciferase Reporter Assay System include:

  1. High sensitivity, wide dynamic range, and flexible applications;

  2. Immediate bioactivity after transcription, without requiring translation;

  3. No species homology between the reporter and control luciferases, avoiding interference;

  4. Firefly and Renilla luciferases use distinct substrates, ensuring no cross-reaction between assays.


Advantages

As a powerful tool for mechanistic studies, dual-luciferase assays are being increasingly applied across a wide range of research areas. Genomeditech offers end-to-end services to support and accelerate your scientific investigations.

Current position:Technical Service > Reporter Gene Detection Services
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Reporter Gene Detection Services
Introduction

A reporter gene is a gene that encodes a protein or enzyme that can be easily identified, and when fused with a target gene, its expression product can be used to monitor the regulation of the target gene's expression. Reporter genes are widely used in studies of gene regulation, RNA interactions, and related fields.


Genomeditech uses the Dual-Luciferase Reporter Assay System to provide reporter gene detection services. This system consists of firefly luciferase and Renilla luciferase, allowing specific transcriptional regulatory elements or binding site sequences to be cloned into a luciferase reporter vector. Changes in luciferase activity are then measured to reflect the transcriptional regulation of the gene of interest. This technique has been widely applied to studies investigating molecular binding mechanisms.



双荧光素酶原理图.png

Features

The Dual-Luciferase Reporter Assay System is a dual-reporter system that uses luciferin and coelenterazine as substrates to detect the activity of firefly luciferase and Renilla luciferase, respectively. Firefly luciferase acts as the reporter gene, catalyzing the oxidation of luciferin into oxyluciferin, a process that emits bioluminescence. Renilla luciferase serves as the internal control, catalyzing the oxidation of coelenterazine into coelenteramide, which also emits bioluminescence.

Bioluminescence produced during the reaction can be measured using a luminometer or a microplate reader to detect changes in luciferase activity.


Key features of the Dual-Luciferase Reporter Assay System include:

  1. High sensitivity, wide dynamic range, and flexible applications;

  2. Immediate bioactivity after transcription, without requiring translation;

  3. No species homology between the reporter and control luciferases, avoiding interference;

  4. Firefly and Renilla luciferases use distinct substrates, ensuring no cross-reaction between assays.


Advantages

As a powerful tool for mechanistic studies, dual-luciferase assays are being increasingly applied across a wide range of research areas. Genomeditech offers end-to-end services to support and accelerate your scientific investigations.

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