Cat. No：GM-C20096
Product：H_LAG3 Reporter Jurkat Cell Line
Cat. No：GM-C20096
Product：H_LAG3 Reporter Jurkat Cell Line
Cell Growth Medium：RPMI 1640+10% FBS+1% P.S+3.5 μg/mL Blasticidin+400 μg/mL G418+0.75 μg/mL Puromycin
Cell Freezing Medium：90% FBS+10% DMSO
Assay Buffer：RPMI 1640+1% FBS+1% P.S
Lymphocyte activation gene-3 (LAG-3; CD223) is a type I transmembrane protein expressed on the surface of activated CD4+ and CD8+ T cells, as well as NK and dendritic cell subpopulations. LAG-3 is closely related to CD4, both molecules having 4 extracellular Ig-like domains, but LAG-3 has a higher affinity for MHC II, approximately 100 times higher than CD4. Traditionally, it is believed that blocking the interaction between LAG-3 and MHC II can restore T cell activity.
The Genomeditech H_LAG3 Reporter Jurkat Cell Line is a luciferase reporter cell line constructed based on the Lag3 signaling pathway. These cells stably express Lag3 gene, luciferase reporter gene, and endogenously express CD4. When cocultured with the Raji Cell Line (Genomeditech/GM-C25385) (endogenously expressing MHC II molecules) and stimulated with sAg, the presence of Lag3 inhibits the binding of MHC II molecules to CD4, thus suppressing T cell signaling. Addition of Anti-Lag3 Ab blocks the interaction between Lag3 and MHC II molecules, thereby restoring T cell signaling. This cell line can be used to study Lag3-related antagonistic drugs.
Cat. No：GM-C20096
Product：H_LAG3 Reporter Jurkat Cell Line
Cell Growth Medium：RPMI 1640+10% FBS+1% P.S+3.5 μg/mL Blasticidin+400 μg/mL G418+0.75 μg/mL Puromycin
Cell Freezing Medium：90% FBS+10% DMSO
Assay Buffer：RPMI 1640+1% FBS+1% P.S
Lymphocyte activation gene-3 (LAG-3; CD223) is a type I transmembrane protein expressed on the surface of activated CD4+ and CD8+ T cells, as well as NK and dendritic cell subpopulations. LAG-3 is closely related to CD4, both molecules having 4 extracellular Ig-like domains, but LAG-3 has a higher affinity for MHC II, approximately 100 times higher than CD4. Traditionally, it is believed that blocking the interaction between LAG-3 and MHC II can restore T cell activity.
The Genomeditech H_LAG3 Reporter Jurkat Cell Line is a luciferase reporter cell line constructed based on the Lag3 signaling pathway. These cells stably express Lag3 gene, luciferase reporter gene, and endogenously express CD4. When cocultured with the Raji Cell Line (Genomeditech/GM-C25385) (endogenously expressing MHC II molecules) and stimulated with sAg, the presence of Lag3 inhibits the binding of MHC II molecules to CD4, thus suppressing T cell signaling. Addition of Anti-Lag3 Ab blocks the interaction between Lag3 and MHC II molecules, thereby restoring T cell signaling. This cell line can be used to study Lag3-related antagonistic drugs.
