Isotype controls are negative controls that help differentiate antibody-specific staining from non-specific background staining in experiments. They are used for accurate detection of antibody drug action and efficacy in both in vitro and in vivo monoclonal antibody (mAb) studies. Isotype controls are most commonly used in FC, IP, and IHC, but can also be used for blocking and coating in other applications such as WB, ELISA, and IF/ICC.
How to choose the appropriate isotype control? Select the same species, the same immunoglobulin type, the same immunoglobulin subclass (for certain species), and the same label as the primary antibody (biotin, HRP, fluorophore, etc.). GenScript develops isotype controls at an in vivo level, providing high-purity monoclonal antibodies with extremely low endotoxin content, as well as sterile, mycoplasma-free, and MVM-free products to serve as a series of references and safeguards for your experiments.
