Cat. No：GM-C21621
Product：H_STING KO THP1 Cell Line
Cat. No：GM-C21621
Product：H_STING KO THP1 Cell Line
Cell Growth Medium：RPMI 1640+10% FBS+1% P.S+0.05 mM β-Me+2 μg/mL Blasticidin+0.5 μg/mL Puromycin
Cell Freezing Medium：90% FBS+10% DMSO
Assay Buffer：RPMI 1640+1% FBS+1% P.S
Gene knockout is the process of using gene editing tools to cut a DNA fragment of a known sequence, causing a double-strand DNA break, and triggering the cell's spontaneous repair mechanism, resulting in the deletion or insertion of bases during repair, causing a frameshift mutation, ultimately achieving the goal of gene knockout.
Based on lentiviral gene editing tools, the H_STING KO THP1 Cell Line constructed by Genomeditech has confirmed DNA-level knockout through Sanger sequencing. Additionally, we have validated protein-level knockout through Western Blot and flow cytometry. All cells are free of mycoplasma contamination, with excellent activity. Our technical experts are available anytime, anywhere to provide cultivation tips and technical advice.
Cat. No：GM-C21621
Product：H_STING KO THP1 Cell Line
Cell Growth Medium：RPMI 1640+10% FBS+1% P.S+0.05 mM β-Me+2 μg/mL Blasticidin+0.5 μg/mL Puromycin
Cell Freezing Medium：90% FBS+10% DMSO
Assay Buffer：RPMI 1640+1% FBS+1% P.S
Gene knockout is the process of using gene editing tools to cut a DNA fragment of a known sequence, causing a double-strand DNA break, and triggering the cell's spontaneous repair mechanism, resulting in the deletion or insertion of bases during repair, causing a frameshift mutation, ultimately achieving the goal of gene knockout.
Based on lentiviral gene editing tools, the H_STING KO THP1 Cell Line constructed by Genomeditech has confirmed DNA-level knockout through Sanger sequencing. Additionally, we have validated protein-level knockout through Western Blot and flow cytometry. All cells are free of mycoplasma contamination, with excellent activity. Our technical experts are available anytime, anywhere to provide cultivation tips and technical advice.
