Cat. No：GM-C21640
Product：STING Reporter THP1 Cell Line
Cat. No：GM-C21640
Product：STING Reporter THP1 Cell Line
Cell Growth Medium：RPMI 1640+10% FBS+1% P.S+0.05 mM 2-Me+2 μg/mL Blasticidin
Cell Freezing Medium：90% FBS+10% DMSO
Assay Buffer：RPMI 1640+1% FBS +1% P.S
Interferon gene stimulator protein (STING) is an intracellular DNA sensor that plays a crucial role in innate immunity by inducing the type I interferon signaling pathway to promote the clearance of pathogens and damaged host cells.
In mammalian cells, this DNA sensing mechanism is mainly mediated by the cGAS-STING signaling pathway (cGAS is cyclic GMP-AMP synthase). The cGAS-STING signaling pathway is critical for inducing inflammation in infection, cellular stress, and tissue damage environments.
Activated STING transmits signals that ultimately induce the production of type I interferons and other inflammatory cytokines, including tumor necrosis factor-alpha (TNF-α). Additionally, the STING signaling pathway is associated with processes like autophagy, cell apoptosis, and necrosis.
The STING Reporter THP1 Cell Line uses THP1 cells as a tool, where stable cell lines expressing reporter genes are established through lentiviral infection. Upon interferon activation, the endogenous interferon-stimulated response element (ISRE) transcription factor binds to DNA response elements, inducing the transcription of a luciferase reporter gene. Apart from interferons, bacteria invading the body typically secrete cyclic dinucleotides (CDNs), which have also been shown to directly bind to STING.
Cat. No：GM-C21640
Product：STING Reporter THP1 Cell Line
Cell Growth Medium：RPMI 1640+10% FBS+1% P.S+0.05 mM 2-Me+2 μg/mL Blasticidin
Cell Freezing Medium：90% FBS+10% DMSO
Assay Buffer：RPMI 1640+1% FBS +1% P.S
Interferon gene stimulator protein (STING) is an intracellular DNA sensor that plays a crucial role in innate immunity by inducing the type I interferon signaling pathway to promote the clearance of pathogens and damaged host cells.
In mammalian cells, this DNA sensing mechanism is mainly mediated by the cGAS-STING signaling pathway (cGAS is cyclic GMP-AMP synthase). The cGAS-STING signaling pathway is critical for inducing inflammation in infection, cellular stress, and tissue damage environments.
Activated STING transmits signals that ultimately induce the production of type I interferons and other inflammatory cytokines, including tumor necrosis factor-alpha (TNF-α). Additionally, the STING signaling pathway is associated with processes like autophagy, cell apoptosis, and necrosis.
The STING Reporter THP1 Cell Line uses THP1 cells as a tool, where stable cell lines expressing reporter genes are established through lentiviral infection. Upon interferon activation, the endogenous interferon-stimulated response element (ISRE) transcription factor binds to DNA response elements, inducing the transcription of a luciferase reporter gene. Apart from interferons, bacteria invading the body typically secrete cyclic dinucleotides (CDNs), which have also been shown to directly bind to STING.
