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Current position:Product Center > Cell lines > Immunotherapy target > STING > H_STING KO U937 Cell Line
H_STING KO U937 Cell Line
Product Info

Cat. No:GM-C21918

Product:H_STING KO U937 Cell Line

Materials required

Cell Growth Medium:RPMI 1640+10% FBS+1% P.S+1 μg/mL Puromycin+25 μg/mL Blasticidin

Cell Freezing Medium:90% FBS+10% DMSO

Assay Buffer:RPMI 1640+1% FBS+1% P.S

Description

Gene knockout is the process of using gene editing tools to cut a DNA fragment of a known sequence, causing a double-strand DNA break, and triggering the cell's spontaneous repair mechanism, resulting in the deletion or insertion of bases during repair, causing a frameshift mutation, ultimately achieving the goal of gene knockout.


Based on lentiviral gene editing tools, the H_STING KO U937 Cell Line constructed by Genomeditech has confirmed DNA-level knockout through Sanger sequencing. Additionally, we have validated protein-level knockout through Western Blot and flow cytometry. All cells are free of mycoplasma contamination, with excellent activity. Our technical experts are available anytime, anywhere to provide cultivation tips and technical advice.

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Current Position:Product Center > Cell lines > Immunotherapy target > STING > H_STING KO U937 Cell Line
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H_STING KO U937 Cell Line
Product Info

Cat. No:GM-C21918

Product:H_STING KO U937 Cell Line

Materials required

Cell Growth Medium:RPMI 1640+10% FBS+1% P.S+1 μg/mL Puromycin+25 μg/mL Blasticidin

Cell Freezing Medium:90% FBS+10% DMSO

Assay Buffer:RPMI 1640+1% FBS+1% P.S

Description

Gene knockout is the process of using gene editing tools to cut a DNA fragment of a known sequence, causing a double-strand DNA break, and triggering the cell's spontaneous repair mechanism, resulting in the deletion or insertion of bases during repair, causing a frameshift mutation, ultimately achieving the goal of gene knockout.


Based on lentiviral gene editing tools, the H_STING KO U937 Cell Line constructed by Genomeditech has confirmed DNA-level knockout through Sanger sequencing. Additionally, we have validated protein-level knockout through Western Blot and flow cytometry. All cells are free of mycoplasma contamination, with excellent activity. Our technical experts are available anytime, anywhere to provide cultivation tips and technical advice.

Data
Message Consultation
If you have more questions, please fill in the relevant information,we'll respond as soon as possible to assist you!
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Submit
You can also contact us on the Scientist and Science Exchange marketplaces.
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