Cat. No：GM-C25209
Product：H_TNFR2 Reporter Jurkat Cell Line
Cat. No：GM-C25209
Product：H_TNFR2 Reporter Jurkat Cell Line
Cell Growth Medium：RPMI 1640+10% FBS+1% P.S+3.5 μg/mL Blasticidin+0.75 μg/mL Puromycin
Cell Freezing Medium：90% FBS+10% DMSO
Assay Buffer：RPMI 1640+1% FBS +1% P.S
Tumor necrosis factor-alpha (TNF-α) is a type II transmembrane protein. It exists in membrane-bound form (mTNF-α). mTNF-α can be processed into 17 kDa soluble TNF-α (sTNF-α) through the action of an enzyme called TNFα-converting enzyme.
TNF-α functions through two TNF receptor superfamily members, type I transmembrane receptor TNF receptor 1 (TNFR1) and TNF receptor 2 (TNFR2). The extracellular domains of both receptors contain similar cysteine-rich motifs, repeated two to six times. TNFR2 (Tumor Necrosis Factor Receptor 2) is mainly activated by mTNF-α and is predominantly expressed in thymic lymphocytes, endothelial cells, microglial cells, and oligodendroglial cells.
The H_TNFR2 Reporter Jurkat Cell Line from Genomeditech is a luciferase reporter cell line constructed based on the TNF signaling pathway. When mTNF-α or antibodies bind to TNFR2, downstream pathways are activated, leading to changes in the expression of luciferase. The luciferase readout represents the activation effect of the signaling pathway and can be used for evaluating the in vitro effects of TNFR2-related drugs. Gibco also offers the H_TNFR2 Reporter V2 Cell Line (Catalog # GM-C25776), in which the TNFR1 gene expressed in the cells has been knocked out, for specific validation experiments targeting TNFR2 antibodies.
| TNF:TNFR2:TNFR1 | ||
| H_TNFR2 Null Reporter Cell Line | H_TNFR2 Reporter V2 Cell Line | Cynomolgus_TNFRSF1B(TNFR2) CHO-K1 Cell Line |
| H_TNFRSF1B(TNFR2) CHO-K1 Cell Line | H_TNFRSF1B(TNFR2) HEK-293 Cell Line | Membrane Bound H_TNFα CHO-K1 Cell Line |
| Membrane Bound H_TNFα(cleavage-resistant) CHO-K1 Cell Line | ||
| Anti-H_TNFR2 hIgG1 Antibody(1H10) | Anti-H_TNFR2 hIgG2 Antibody(1) | Anti-H_TNFRSF1B(TNFR2) hIgG1 Antibody(UC2.3.8) |
| Anti-TNFR1 hIgG1 Antibody(Atrosab) | Anti-TNF-α hIgG1 Antibody（CT-P17） | |
Cat. No：GM-C25209
Product：H_TNFR2 Reporter Jurkat Cell Line
Cell Growth Medium：RPMI 1640+10% FBS+1% P.S+3.5 μg/mL Blasticidin+0.75 μg/mL Puromycin
Cell Freezing Medium：90% FBS+10% DMSO
Assay Buffer：RPMI 1640+1% FBS +1% P.S
Tumor necrosis factor-alpha (TNF-α) is a type II transmembrane protein. It exists in membrane-bound form (mTNF-α). mTNF-α can be processed into 17 kDa soluble TNF-α (sTNF-α) through the action of an enzyme called TNFα-converting enzyme.
TNF-α functions through two TNF receptor superfamily members, type I transmembrane receptor TNF receptor 1 (TNFR1) and TNF receptor 2 (TNFR2). The extracellular domains of both receptors contain similar cysteine-rich motifs, repeated two to six times. TNFR2 (Tumor Necrosis Factor Receptor 2) is mainly activated by mTNF-α and is predominantly expressed in thymic lymphocytes, endothelial cells, microglial cells, and oligodendroglial cells.
The H_TNFR2 Reporter Jurkat Cell Line from Genomeditech is a luciferase reporter cell line constructed based on the TNF signaling pathway. When mTNF-α or antibodies bind to TNFR2, downstream pathways are activated, leading to changes in the expression of luciferase. The luciferase readout represents the activation effect of the signaling pathway and can be used for evaluating the in vitro effects of TNFR2-related drugs. Gibco also offers the H_TNFR2 Reporter V2 Cell Line (Catalog # GM-C25776), in which the TNFR1 gene expressed in the cells has been knocked out, for specific validation experiments targeting TNFR2 antibodies.
| TNF:TNFR2:TNFR1 | ||
| H_TNFR2 Null Reporter Cell Line | H_TNFR2 Reporter V2 Cell Line | Cynomolgus_TNFRSF1B(TNFR2) CHO-K1 Cell Line |
| H_TNFRSF1B(TNFR2) CHO-K1 Cell Line | H_TNFRSF1B(TNFR2) HEK-293 Cell Line | Membrane Bound H_TNFα CHO-K1 Cell Line |
| Membrane Bound H_TNFα(cleavage-resistant) CHO-K1 Cell Line | ||
| Anti-H_TNFR2 hIgG1 Antibody(1H10) | Anti-H_TNFR2 hIgG2 Antibody(1) | Anti-H_TNFRSF1B(TNFR2) hIgG1 Antibody(UC2.3.8) |
| Anti-TNFR1 hIgG1 Antibody(Atrosab) | Anti-TNF-α hIgG1 Antibody（CT-P17） | |
