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Current position:Product Center > Cell lines > Immunotherapy target > BTN3:Vγ9Vδ2 > TCR Knockout Jurkat Cell Line
TCR Knockout Jurkat Cell Line
Product Info

Cat. No:GM-C19594

Product:TCR Knockout Jurkat Cell Line


Materials required

Cell Growth Medium:RPMI 1640+10% FBS+1% P.S+400 μg/mL G418+200 μg/mL Hygromycin

Cell Freezing Medium:90% FBS+10% DMSO

Description

The knockout of genes is achieved by using gene editing tools to cut a known DNA sequence, causing DNA double-strand breaks and triggering the cell's intrinsic repair mechanism. This leads to the deletion or insertion of bases during repair, resulting in a frameshift mutation and ultimately achieving the goal of gene knockout.


Based on the slow virus gene editing tool, Genomeditech has developed the TCR Knockout Jurkat Cell Line, confirmed the knockout at the DNA level through Sanger sequencing, and validated the knockout at the protein level through Western Blot and flow cytometry. All cells are free of mycoplasma and exhibit good activity. Our technical experts are available anytime, anywhere to provide you with cultivation techniques and technical advice.

Data
Current Position:Product Center > Cell lines > Immunotherapy target > BTN3:Vγ9Vδ2 > TCR Knockout Jurkat Cell Line
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TCR Knockout Jurkat Cell Line
Product Info

Cat. No:GM-C19594

Product:TCR Knockout Jurkat Cell Line


Materials required

Cell Growth Medium:RPMI 1640+10% FBS+1% P.S+400 μg/mL G418+200 μg/mL Hygromycin

Cell Freezing Medium:90% FBS+10% DMSO

Description

The knockout of genes is achieved by using gene editing tools to cut a known DNA sequence, causing DNA double-strand breaks and triggering the cell's intrinsic repair mechanism. This leads to the deletion or insertion of bases during repair, resulting in a frameshift mutation and ultimately achieving the goal of gene knockout.


Based on the slow virus gene editing tool, Genomeditech has developed the TCR Knockout Jurkat Cell Line, confirmed the knockout at the DNA level through Sanger sequencing, and validated the knockout at the protein level through Western Blot and flow cytometry. All cells are free of mycoplasma and exhibit good activity. Our technical experts are available anytime, anywhere to provide you with cultivation techniques and technical advice.

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