Cat. No：GM-C23900
Product：H_SIRPα Blockade Reporter Cell Line
Cat. No：GM-C23900
Product：H_SIRPα Blockade Reporter Cell Line
Cell Growth Medium：RPMI 1640+10% FBS+1% P.S+400 μg/mL G418+0.75 μg/mL Puromycin+200 μg/mL Hygromycin+3.5 μg/mL Blasticidin
Cell Freezing Medium：90% FBS+10% DMSO
Assay Buffer：RPMI 1640+1% FBS+1% P.S
CD47, known as Integrin-Associated Protein (IAP), is a transmembrane protein encoded by the CD47 gene in the human body, belonging to the immunoglobulin superfamily. It interacts with integrins, Thrombospondin-1 (TSP-1), and Signal Regulatory Protein alpha (Sirpα). Its protein structure consists of an extracellular N-terminal IgV domain, 5 transmembrane domains, and an intracellular C-terminal transmembrane glycoprotein, widely expressed on the surface of almost all normal cells.
Sirpα is a membrane glycoprotein from the SIRP family, predominantly expressed on macrophages, dendritic cells, and neural cells. As an inhibitory receptor, it interacts with CD47, releasing a "do not eat me" signal, thereby inhibiting macrophage engulfment. Some tumor cells also overexpress CD47 to escape the immune system. The cytoplasmic region of SIRPα is highly conserved between humans, rats, and mice, containing numerous tyrosine residues ITIM. When binding with CD47, Sirpα is phosphorylated and recruits SHP1 and SHP2, transmitting intracellular signals.
The Genomeditech H_SIRPα Blockade Reporter Cell Line is a reporter cell line that activates CD3ζ and subsequently luciferase expression upon CD47 binding to SIRPα. This activation can be blocked by antibodies with a blocking effect, making this cell model suitable for screening blocking antibodies. Luciferase readings represent the activation of the signaling pathway and the blocking effect, thereby allowing for the evaluation of the in vitro effects of CD47 and SIRPα-related antibody drugs.
| CD47:SIRPα | ||
| CD47 Knockout Jurkat Cell Line | H_SIRPα Reporter Jurkat Cell Line | Cynomolgus_CD47 CHO-K1 Cell Line |
| H_CD47 aAPC CHO-K1 Cell Line | H_CD47 CHO-K1 cell line | H_CD47 LLC1 cell line |
| H_CD47 MC38 Cell Line | H_CD47 PDL1 MC38(mouse_PDL1 KO) Cell Line | H_SIRPA(SIRPα) CHO-K1 Cell Line |
| Mouse_CD47 CHO-K1 Cell Line | ||
| Anti-CD47 hIgG4 Antibody(5F9) | Anti-H_SIRPα hIgG1 Antibody | Anti-H_SIRPα hIgG1 Antibody(Hu1H9-G1) |
| Anti-mouse SIRPA RlgG1 Antibody(p84) | ||
Cat. No：GM-C23900
Product：H_SIRPα Blockade Reporter Cell Line
Cell Growth Medium：RPMI 1640+10% FBS+1% P.S+400 μg/mL G418+0.75 μg/mL Puromycin+200 μg/mL Hygromycin+3.5 μg/mL Blasticidin
Cell Freezing Medium：90% FBS+10% DMSO
Assay Buffer：RPMI 1640+1% FBS+1% P.S
CD47, known as Integrin-Associated Protein (IAP), is a transmembrane protein encoded by the CD47 gene in the human body, belonging to the immunoglobulin superfamily. It interacts with integrins, Thrombospondin-1 (TSP-1), and Signal Regulatory Protein alpha (Sirpα). Its protein structure consists of an extracellular N-terminal IgV domain, 5 transmembrane domains, and an intracellular C-terminal transmembrane glycoprotein, widely expressed on the surface of almost all normal cells.
Sirpα is a membrane glycoprotein from the SIRP family, predominantly expressed on macrophages, dendritic cells, and neural cells. As an inhibitory receptor, it interacts with CD47, releasing a "do not eat me" signal, thereby inhibiting macrophage engulfment. Some tumor cells also overexpress CD47 to escape the immune system. The cytoplasmic region of SIRPα is highly conserved between humans, rats, and mice, containing numerous tyrosine residues ITIM. When binding with CD47, Sirpα is phosphorylated and recruits SHP1 and SHP2, transmitting intracellular signals.
The Genomeditech H_SIRPα Blockade Reporter Cell Line is a reporter cell line that activates CD3ζ and subsequently luciferase expression upon CD47 binding to SIRPα. This activation can be blocked by antibodies with a blocking effect, making this cell model suitable for screening blocking antibodies. Luciferase readings represent the activation of the signaling pathway and the blocking effect, thereby allowing for the evaluation of the in vitro effects of CD47 and SIRPα-related antibody drugs.
| CD47:SIRPα | ||
| CD47 Knockout Jurkat Cell Line | H_SIRPα Reporter Jurkat Cell Line | Cynomolgus_CD47 CHO-K1 Cell Line |
| H_CD47 aAPC CHO-K1 Cell Line | H_CD47 CHO-K1 cell line | H_CD47 LLC1 cell line |
| H_CD47 MC38 Cell Line | H_CD47 PDL1 MC38(mouse_PDL1 KO) Cell Line | H_SIRPA(SIRPα) CHO-K1 Cell Line |
| Mouse_CD47 CHO-K1 Cell Line | ||
| Anti-CD47 hIgG4 Antibody(5F9) | Anti-H_SIRPα hIgG1 Antibody | Anti-H_SIRPα hIgG1 Antibody(Hu1H9-G1) |
| Anti-mouse SIRPA RlgG1 Antibody(p84) | ||
