Cat. No：GM-C26024
Product：ADCC FcγRIIIa(158V) DDX35TM Jurkat Effector Cell Line
Cat. No：GM-C26024
Product：ADCC FcγRIIIa(158V) DDX35TM Jurkat Effector Cell Line
Cell Growth Medium：RPMI 1640+10% FBS+1% P.S+3.5 μg/mL Blasticidin+0.75 μg/mL Puromycin
Cell Freezing Medium：90% FBS+10% DMSO
RPMI 1640+10% FBS+1% P.S+3.5 μg/mL Blasticidin+0.75 μg/mL Puromycin
ADCC, or antibody-dependent cell-mediated cytotoxicity, refers to the cytotoxic effect mediated by immune cells expressing Fc receptors which directly kill target cells by recognizing the Fc portion of antibodies that bind specifically to them. Today, the mechanism of ADCC is used to detect and assess the efficacy of antibodies or target cells. The killing of target cells is the endpoint of this activation pathway, used in classical ADCC bioactivity assays.
The ADCC FcγRIIIa(158V) DDX35TM Jurkat Effector Cell Line from Genomeditech employs engineered Jurkat cells as effector cells, stably expressing the human FcγRIIIa receptor (158V) and firefly luciferase driven by transcription factors. The biological activity of antibodies in ADCC is quantified through luminescence produced by the NFAT pathway activation, and effector cell luminescence activity is quantified by bioluminescence readings. This method provides high signal values with low background and offers customization of ADCC cell lines and ADCC activity detection services.
| FcγR | ||
| Cynomolgus_FcRn MDCK Cell Line | H_FCGR1A(CD64) CHO-K1 Cell Line | H_FCGR1A(CD64) HEK-293 Cell Line |
| H_FCGR2A(CD32A) CHO-K1 Cell Line | H_FCGR2B(CD32B) CHO-K1 Cell Line | H_FCGR3A(CD16a) 158F CHO-K1 Cell Line |
| H_FCGR3A(CD16a) 158V CHO-K1 Cell Line | H_FCGR3B(CD16b) CHO-K1 Cell Line | H_FcRn CHO-K1 Cell Line |
| H_FcRn MDCK Cell Line | Mouse_FcRn MDCK Cell Line | |
| Anti-FcRn hIgG4 Reference Antibody(Rozabio) | Anti-H_FcRn IgG4 Antibody(Rozanolixizumab) | Anti-Mouse CD1632 mIgG2b Antibody(2.4G2) |
| ADCCP | ||
| ADCC FcγRIIIa(158F) Jurkat Effector Cell Line | ADCC FcγRIIIa(158V) Jurkat Effector Cell Line | ADCC M_FcγRIV Jurkat Effector Cell Line |
| ADCP FcγRI Jurkat Effector Cell Line | ADCP FcγRIIa DDX35TM Jurkat Effector Cell Line | ADCP FcγRIIa Jurkat Effector Cell Line |
| ADCP FcγRIIa R131 Jurkat Effector Cell Line | ADCP FcγRIIb Jurkat Effector Cell Line | |
Cat. No：GM-C26024
Product：ADCC FcγRIIIa(158V) DDX35TM Jurkat Effector Cell Line
Cell Growth Medium：RPMI 1640+10% FBS+1% P.S+3.5 μg/mL Blasticidin+0.75 μg/mL Puromycin
Cell Freezing Medium：90% FBS+10% DMSO
RPMI 1640+10% FBS+1% P.S+3.5 μg/mL Blasticidin+0.75 μg/mL Puromycin
ADCC, or antibody-dependent cell-mediated cytotoxicity, refers to the cytotoxic effect mediated by immune cells expressing Fc receptors which directly kill target cells by recognizing the Fc portion of antibodies that bind specifically to them. Today, the mechanism of ADCC is used to detect and assess the efficacy of antibodies or target cells. The killing of target cells is the endpoint of this activation pathway, used in classical ADCC bioactivity assays.
The ADCC FcγRIIIa(158V) DDX35TM Jurkat Effector Cell Line from Genomeditech employs engineered Jurkat cells as effector cells, stably expressing the human FcγRIIIa receptor (158V) and firefly luciferase driven by transcription factors. The biological activity of antibodies in ADCC is quantified through luminescence produced by the NFAT pathway activation, and effector cell luminescence activity is quantified by bioluminescence readings. This method provides high signal values with low background and offers customization of ADCC cell lines and ADCC activity detection services.
| FcγR | ||
| Cynomolgus_FcRn MDCK Cell Line | H_FCGR1A(CD64) CHO-K1 Cell Line | H_FCGR1A(CD64) HEK-293 Cell Line |
| H_FCGR2A(CD32A) CHO-K1 Cell Line | H_FCGR2B(CD32B) CHO-K1 Cell Line | H_FCGR3A(CD16a) 158F CHO-K1 Cell Line |
| H_FCGR3A(CD16a) 158V CHO-K1 Cell Line | H_FCGR3B(CD16b) CHO-K1 Cell Line | H_FcRn CHO-K1 Cell Line |
| H_FcRn MDCK Cell Line | Mouse_FcRn MDCK Cell Line | |
| Anti-FcRn hIgG4 Reference Antibody(Rozabio) | Anti-H_FcRn IgG4 Antibody(Rozanolixizumab) | Anti-Mouse CD1632 mIgG2b Antibody(2.4G2) |
| ADCCP | ||
| ADCC FcγRIIIa(158F) Jurkat Effector Cell Line | ADCC FcγRIIIa(158V) Jurkat Effector Cell Line | ADCC M_FcγRIV Jurkat Effector Cell Line |
| ADCP FcγRI Jurkat Effector Cell Line | ADCP FcγRIIa DDX35TM Jurkat Effector Cell Line | ADCP FcγRIIa Jurkat Effector Cell Line |
| ADCP FcγRIIa R131 Jurkat Effector Cell Line | ADCP FcγRIIb Jurkat Effector Cell Line | |
