Cat. No：GM-C01619
Product：ADCC FcγRIIIa(158F) Jurkat Effector Cell Line
Cat. No：GM-C01619
Product：ADCC FcγRIIIa(158F) Jurkat Effector Cell Line
Cell Growth Medium：RPMI-1640+10%FBS+1%P.S+0.75 μg/mL Puromycin+3.5 μg/mL Blasticidin
Cell Freezing Medium：FBS+10% DMSO
ADCC detection buffer： 99% RPMI1640+1% low IgG FBS
ADCC, which stands for antibody-dependent cell-mediated cytotoxicity, refers to the mechanism in which immune cells expressing Fc receptors directly kill target cells that are bound by antibodies through the Fc segment recognition. Nowadays, the ADCC mechanism is used to assess the efficacy of antibodies or target cells. The killing of target cells is the endpoint of this activation pathway and is utilized in classical ADCC bioactivity assays. These assays typically use peripheral blood mononuclear cells (PBMC) or natural killer (NK) cell subsets from donors as effector cells. These cells exhibit significant variability in response, are challenging to prepare, and can lead to high background readings.
ADCC Reporter cell lines focus on early events in the ADCC mechanism pathway activation as an indicator for detection: specifically, the activation of gene transcription mediated by the NFAT (nuclear factor of activated T-cells) pathway in effector cells. Moreover, the ADCC reporter gene assay kit uses engineered Jurkat cells as effector cells, which stably express the FcγRIIIa receptor (158F) and firefly luciferase driven by NFAT response elements. The bioactivity of antibodies in the ADCC mechanism is quantified based on the luminescence generated through NFAT pathway activation, while the luminescence activity in effector cells is quantified through bioluminescence readings. The detected signal is high, and the background is low.
Genomeditech relies on years of research experience, utilizing clever vector design and a third-generation lentiviral reporter gene system to launch ADCC-related cell lines and ADCC activity detection services, as well as offering customized ADCC cell line services.
| FcγR | ||
| Cynomolgus_FcRn MDCK Cell Line | H_FCGR1A(CD64) CHO-K1 Cell Line | H_FCGR1A(CD64) HEK-293 Cell Line |
| H_FCGR2A(CD32A) CHO-K1 Cell Line | H_FCGR2B(CD32B) CHO-K1 Cell Line | H_FCGR3A(CD16a) 158F CHO-K1 Cell Line |
| H_FCGR3A(CD16a) 158V CHO-K1 Cell Line | H_FCGR3B(CD16b) CHO-K1 Cell Line | H_FcRn CHO-K1 Cell Line |
| H_FcRn MDCK Cell Line | Mouse_FcRn MDCK Cell Line | |
| Anti-FcRn hIgG4 Reference Antibody(Rozabio) | Anti-H_FcRn IgG4 Antibody(Rozanolixizumab) | Anti-Mouse CD1632 mIgG2b Antibody(2.4G2) |
| ADCCP | ||
| ADCC FcγRIIIa(158V) DDX35TM Jurkat Effector Cell Line | ADCC FcγRIIIa(158V) Jurkat Effector Cell Line | ADCC M_FcγRIV Jurkat Effector Cell Line |
| ADCP FcγRI Jurkat Effector Cell Line | ADCP FcγRIIa DDX35TM Jurkat Effector Cell Line | ADCP FcγRIIa Jurkat Effector Cell Line |
| ADCP FcγRIIa R131 Jurkat Effector Cell Line | ADCP FcγRIIb Jurkat Effector Cell Line | |
Cat. No：GM-C01619
Product：ADCC FcγRIIIa(158F) Jurkat Effector Cell Line
Cell Growth Medium：RPMI-1640+10%FBS+1%P.S+0.75 μg/mL Puromycin+3.5 μg/mL Blasticidin
Cell Freezing Medium：FBS+10% DMSO
ADCC detection buffer： 99% RPMI1640+1% low IgG FBS
ADCC, which stands for antibody-dependent cell-mediated cytotoxicity, refers to the mechanism in which immune cells expressing Fc receptors directly kill target cells that are bound by antibodies through the Fc segment recognition. Nowadays, the ADCC mechanism is used to assess the efficacy of antibodies or target cells. The killing of target cells is the endpoint of this activation pathway and is utilized in classical ADCC bioactivity assays. These assays typically use peripheral blood mononuclear cells (PBMC) or natural killer (NK) cell subsets from donors as effector cells. These cells exhibit significant variability in response, are challenging to prepare, and can lead to high background readings.
ADCC Reporter cell lines focus on early events in the ADCC mechanism pathway activation as an indicator for detection: specifically, the activation of gene transcription mediated by the NFAT (nuclear factor of activated T-cells) pathway in effector cells. Moreover, the ADCC reporter gene assay kit uses engineered Jurkat cells as effector cells, which stably express the FcγRIIIa receptor (158F) and firefly luciferase driven by NFAT response elements. The bioactivity of antibodies in the ADCC mechanism is quantified based on the luminescence generated through NFAT pathway activation, while the luminescence activity in effector cells is quantified through bioluminescence readings. The detected signal is high, and the background is low.
Genomeditech relies on years of research experience, utilizing clever vector design and a third-generation lentiviral reporter gene system to launch ADCC-related cell lines and ADCC activity detection services, as well as offering customized ADCC cell line services.
| FcγR | ||
| Cynomolgus_FcRn MDCK Cell Line | H_FCGR1A(CD64) CHO-K1 Cell Line | H_FCGR1A(CD64) HEK-293 Cell Line |
| H_FCGR2A(CD32A) CHO-K1 Cell Line | H_FCGR2B(CD32B) CHO-K1 Cell Line | H_FCGR3A(CD16a) 158F CHO-K1 Cell Line |
| H_FCGR3A(CD16a) 158V CHO-K1 Cell Line | H_FCGR3B(CD16b) CHO-K1 Cell Line | H_FcRn CHO-K1 Cell Line |
| H_FcRn MDCK Cell Line | Mouse_FcRn MDCK Cell Line | |
| Anti-FcRn hIgG4 Reference Antibody(Rozabio) | Anti-H_FcRn IgG4 Antibody(Rozanolixizumab) | Anti-Mouse CD1632 mIgG2b Antibody(2.4G2) |
| ADCCP | ||
| ADCC FcγRIIIa(158V) DDX35TM Jurkat Effector Cell Line | ADCC FcγRIIIa(158V) Jurkat Effector Cell Line | ADCC M_FcγRIV Jurkat Effector Cell Line |
| ADCP FcγRI Jurkat Effector Cell Line | ADCP FcγRIIa DDX35TM Jurkat Effector Cell Line | ADCP FcγRIIa Jurkat Effector Cell Line |
| ADCP FcγRIIa R131 Jurkat Effector Cell Line | ADCP FcγRIIb Jurkat Effector Cell Line | |
