Cat. No：GM-C09105
Product：ADCC M_FcγRIV Jurkat Effector Cell Line
Cat. No：GM-C09105
Product：ADCC M_FcγRIV Jurkat Effector Cell Line
Cell Growth Medium：1640+10%FBS+1%P.S+3.5ug/ml Blasticidin+0.75ug/ml Puromycin
Cell Freezing Medium：FBS+10% DMSO
Assay Buffer：1640+1% low IgG FBS
ADCC, or antibody-dependent cell-mediated cytotoxicity, refers to the cytotoxic effect mediated by immune cells expressing Fc receptors, which directly kill target cells by recognizing the Fc segment of antibodies bound to them. Today, the ADCC mechanism is used to assess the efficacy of antibodies or target cells. The activation of the ADCC pathway occurs when antibodies bind to target antigens on cell surfaces and simultaneously bind to the FcγRIIIa receptor on effector cells, primarily natural killer cells, leading to the activation of the ADCC mechanism. The killing of target cells is the endpoint of this activation pathway, commonly used in classic ADCC bioactivity assays.
ADCC Reporter cell lines measure the early event of NFAT-mediated gene transcription activation in effector cells as a readout indicator. These reporter gene assay kits utilize engineered Jurkat cells expressing stable FcγRIIIa receptors (high-affinity 158V) and firefly luciferase driven by NFAT response elements in effector cells. The biological activity of antibodies in the ADCC mechanism is quantified by the luminescence produced through NFAT pathway activation, with luminescence activity in effector cells quantified by bioluminescence readings. The signal detection is high, with low background noise.
With years of research experience,Genomeditech offers ADCC-related cell lines and activity testing services using clever vector design and third-generation lentiviral reporter gene systems, including customized ADCC cell line services.
| FcγR | ||
| Cynomolgus_FcRn MDCK Cell Line | H_FCGR1A(CD64) CHO-K1 Cell Line | H_FCGR1A(CD64) HEK-293 Cell Line |
| H_FCGR2A(CD32A) CHO-K1 Cell Line | H_FCGR2B(CD32B) CHO-K1 Cell Line | H_FCGR3A(CD16a) 158F CHO-K1 Cell Line |
| H_FCGR3A(CD16a) 158V CHO-K1 Cell Line | H_FCGR3B(CD16b) CHO-K1 Cell Line | H_FcRn CHO-K1 Cell Line |
| H_FcRn MDCK Cell Line | Mouse_FcRn MDCK Cell Line | |
| Anti-FcRn hIgG4 Reference Antibody(Rozabio) | Anti-H_FcRn IgG4 Antibody(Rozanolixizumab) | Anti-Mouse CD1632 mIgG2b Antibody(2.4G2) |
| ADCCP | ||
| ADCC FcγRIIIa(158F) Jurkat Effector Cell Line | ADCC FcγRIIIa(158V) DDX35TM Jurkat Effector Cell Line | ADCC FcγRIIIa(158V) Jurkat Effector Cell Line |
| ADCP FcγRI Jurkat Effector Cell Line | ADCP FcγRIIa DDX35TM Jurkat Effector Cell Line | ADCP FcγRIIa Jurkat Effector Cell Line |
| ADCP FcγRIIa R131 Jurkat Effector Cell Line | ADCP FcγRIIb Jurkat Effector Cell Line | |
Cat. No：GM-C09105
Product：ADCC M_FcγRIV Jurkat Effector Cell Line
Cell Growth Medium：1640+10%FBS+1%P.S+3.5ug/ml Blasticidin+0.75ug/ml Puromycin
Cell Freezing Medium：FBS+10% DMSO
Assay Buffer：1640+1% low IgG FBS
ADCC, or antibody-dependent cell-mediated cytotoxicity, refers to the cytotoxic effect mediated by immune cells expressing Fc receptors, which directly kill target cells by recognizing the Fc segment of antibodies bound to them. Today, the ADCC mechanism is used to assess the efficacy of antibodies or target cells. The activation of the ADCC pathway occurs when antibodies bind to target antigens on cell surfaces and simultaneously bind to the FcγRIIIa receptor on effector cells, primarily natural killer cells, leading to the activation of the ADCC mechanism. The killing of target cells is the endpoint of this activation pathway, commonly used in classic ADCC bioactivity assays.
ADCC Reporter cell lines measure the early event of NFAT-mediated gene transcription activation in effector cells as a readout indicator. These reporter gene assay kits utilize engineered Jurkat cells expressing stable FcγRIIIa receptors (high-affinity 158V) and firefly luciferase driven by NFAT response elements in effector cells. The biological activity of antibodies in the ADCC mechanism is quantified by the luminescence produced through NFAT pathway activation, with luminescence activity in effector cells quantified by bioluminescence readings. The signal detection is high, with low background noise.
With years of research experience,Genomeditech offers ADCC-related cell lines and activity testing services using clever vector design and third-generation lentiviral reporter gene systems, including customized ADCC cell line services.
| FcγR | ||
| Cynomolgus_FcRn MDCK Cell Line | H_FCGR1A(CD64) CHO-K1 Cell Line | H_FCGR1A(CD64) HEK-293 Cell Line |
| H_FCGR2A(CD32A) CHO-K1 Cell Line | H_FCGR2B(CD32B) CHO-K1 Cell Line | H_FCGR3A(CD16a) 158F CHO-K1 Cell Line |
| H_FCGR3A(CD16a) 158V CHO-K1 Cell Line | H_FCGR3B(CD16b) CHO-K1 Cell Line | H_FcRn CHO-K1 Cell Line |
| H_FcRn MDCK Cell Line | Mouse_FcRn MDCK Cell Line | |
| Anti-FcRn hIgG4 Reference Antibody(Rozabio) | Anti-H_FcRn IgG4 Antibody(Rozanolixizumab) | Anti-Mouse CD1632 mIgG2b Antibody(2.4G2) |
| ADCCP | ||
| ADCC FcγRIIIa(158F) Jurkat Effector Cell Line | ADCC FcγRIIIa(158V) DDX35TM Jurkat Effector Cell Line | ADCC FcγRIIIa(158V) Jurkat Effector Cell Line |
| ADCP FcγRI Jurkat Effector Cell Line | ADCP FcγRIIa DDX35TM Jurkat Effector Cell Line | ADCP FcγRIIa Jurkat Effector Cell Line |
| ADCP FcγRIIa R131 Jurkat Effector Cell Line | ADCP FcγRIIb Jurkat Effector Cell Line | |
