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Current Position： Product Center > Cell Lines > Immune Checkpoint > H_PDCD1LG2(PDL2) aAPC CHO-K1 Cell Line
H_PDCD1LG2(PDL2) aAPC CHO-K1 Cell Line
Manual
Cat. No.
GM-C31280
Size
1 vial
Quote
Product Info
Data display
Materials required
Description
Related products
Product Info

Cat. No：GM-C31280

Product：H_PDCD1LG2(PDL2) aAPC CHO-K1 Cell Line


产品配图.jpg

Data display
H_PDCD1LG2(PDL2) aAPC CHO-K1 Cell Line (Cat. GM-C31280) was determined by flow cytometry using Anti-PD-L2 hIgG1 Antibody(Hz25G4-1.1) (Cat. GM-79817AB).
Response to Anti-PD1 hIgG4 Antibody(Pembrolizumab). H_PDCD1LG2(PDL2) aAPC CHO-K1 Cell Line (Cat. GM-C31280) was seeded at a density of 1E4 cells per well in a 96-well plate and incubated overnight. The next day, serial dilutions of the Anti-PD1 hIgG4 Antibody(Pembrolizumab) (Cat. GM-52674AB) were incubated with 1E5 cells/well of the H_PD-1 Reporter Jurkat Cell Line (Cat. GM-C07928) in a 96-well plate for 1 hour, and then added to the pre-seeded cells. The mixture was incubated for an additional 16 hours. Firefly luciferase activity was then measured using the GMOne-Step Luciferase Reporter Gene Assay Kit (Cat. GM-040503). Data are shown by drug mass concentration.
The passage stability of the H_PDCD1LG2(PDL2) aAPC CHO-K1 Cell Line (Cat. GM-C31280) was determined by flow cytometry using Anti-PD-L2 hIgG1 Antibody(Hz25G4-1.1) (Cat. GM-79817AB).
The passage stability of response to Anti-PD1 hIgG4 Antibody(Pembrolizumab). Incubate the CP6、 CP11、CP16、CP21 and CP26 passage of 1E5 cells/well H_PD-1 Reporter Jurkat Cell Line (Cat. GM-C07928) with serial dilutions of the Anti-PD1 hIgG4 Antibody(Pembrolizumab) (Cat. GM-52674AB) for 1 hour, then, add the mixture to the 1E4 cells/well of the H_PDCD1LG2(PDL2) aAPC CHO-K1 Cell Line (Cat. GM-C31280), which was prepared overnight, and incubate for 16 hours. The firefly luciferase activity was measured using the GMOne-Step Luciferase Reporter Gene Assay Kit (Cat. GM-040503). Data are shown by drug mass concentration.
Materials required

Cell Growth Medium：F12K+10% FBS+1% P.S+4 μg/mL Blasticidin+4 μg/mL Puromycin

Cell Freezing Medium：90% FBS+10% DMSO

Description


H_PDCD1LG2(PDL2) Amino acid sequence (Q9BQ51-1)：

MIFLLLMLSLELQLHQIAALFTVTVPKELYIIEHGSNVTLECNFDTGSHVNLGAITASLQKVENDTSPHRERATLLEEQLPLGKASFHIPQVQVRDEGQYQCIIIYGVAWDYKYLTLKVKASYRKINTHILKVPETDEVELTCQATGYPLAEVSWPNVSVPANTSHSRTPEGLYQVTSVLRLKPPPGRNFSCVFWNTHVRELTLASIDLQSQMEPRTHPTWLLHIFIPFCIIAFIFIATVIALRKQLCQKLYSSKDTTKRPVTTTKREVNSAI

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Anti-H_PDCD1(PD1) hIgG1 Antibody(Budigalimab)Anti-H_PDCD1LG2 mIgG1 Antibody(3G2)Anti-Mouse PD-L1 mIgG1 Antibody(10F.9G2)
Anti-Mouse_PD1 mIgG1 AntibodyAnti-PD1 hIgG4 Antibody(Pembrolizumab)Anti-PD1 hIgG4 Reference Antibody (Nivbio)
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Anti-PD-L2 hIgG1 Antibody(Hz25G4-1.1)

Biotinylated Cynomolgus PDL1 Protein; His-Avi TagBiotinylated Human PD1 Protein; His-Avi TagBiotinylated Human PDL1 Protein; His-Avi Tag
Canine PD1 Protein; hFc TagCanine PDL1 Protein; hFc TagCanine PDL1 Protein; His Tag
Cynomolgus PDL1 Protein; His TagHuman PD1 Protein; His TagHuman PDL1 Protein; His Tag
H_PDCD1LG2(PDL2) aAPC CHO-K1 Cell Line
Manual
Cat. No.
GM-C31280
Size
1 vial
Quote
Product Info
Data display
Materials required
Description
Related products
Product Info

Cat. No：GM-C31280

Product：H_PDCD1LG2(PDL2) aAPC CHO-K1 Cell Line


产品配图.jpg

Cat. No：GM-C31280

Product：H_PDCD1LG2(PDL2) aAPC CHO-K1 Cell Line


产品配图.jpg

Data display
H_PDCD1LG2(PDL2) aAPC CHO-K1 Cell Line (Cat. GM-C31280) was determined by flow cytometry using Anti-PD-L2 hIgG1 Antibody(Hz25G4-1.1) (Cat. GM-79817AB).
Response to Anti-PD1 hIgG4 Antibody(Pembrolizumab). H_PDCD1LG2(PDL2) aAPC CHO-K1 Cell Line (Cat. GM-C31280) was seeded at a density of 1E4 cells per well in a 96-well plate and incubated overnight. The next day, serial dilutions of the Anti-PD1 hIgG4 Antibody(Pembrolizumab) (Cat. GM-52674AB) were incubated with 1E5 cells/well of the H_PD-1 Reporter Jurkat Cell Line (Cat. GM-C07928) in a 96-well plate for 1 hour, and then added to the pre-seeded cells. The mixture was incubated for an additional 16 hours. Firefly luciferase activity was then measured using the GMOne-Step Luciferase Reporter Gene Assay Kit (Cat. GM-040503). Data are shown by drug mass concentration.
The passage stability of the H_PDCD1LG2(PDL2) aAPC CHO-K1 Cell Line (Cat. GM-C31280) was determined by flow cytometry using Anti-PD-L2 hIgG1 Antibody(Hz25G4-1.1) (Cat. GM-79817AB).
The passage stability of response to Anti-PD1 hIgG4 Antibody(Pembrolizumab). Incubate the CP6、 CP11、CP16、CP21 and CP26 passage of 1E5 cells/well H_PD-1 Reporter Jurkat Cell Line (Cat. GM-C07928) with serial dilutions of the Anti-PD1 hIgG4 Antibody(Pembrolizumab) (Cat. GM-52674AB) for 1 hour, then, add the mixture to the 1E4 cells/well of the H_PDCD1LG2(PDL2) aAPC CHO-K1 Cell Line (Cat. GM-C31280), which was prepared overnight, and incubate for 16 hours. The firefly luciferase activity was measured using the GMOne-Step Luciferase Reporter Gene Assay Kit (Cat. GM-040503). Data are shown by drug mass concentration.
Materials required

Cell Growth Medium：F12K+10% FBS+1% P.S+4 μg/mL Blasticidin+4 μg/mL Puromycin

Cell Freezing Medium：90% FBS+10% DMSO

Cell Growth Medium：F12K+10% FBS+1% P.S+4 μg/mL Blasticidin+4 μg/mL Puromycin

Cell Freezing Medium：90% FBS+10% DMSO

Description


H_PDCD1LG2(PDL2) Amino acid sequence (Q9BQ51-1)：

MIFLLLMLSLELQLHQIAALFTVTVPKELYIIEHGSNVTLECNFDTGSHVNLGAITASLQKVENDTSPHRERATLLEEQLPLGKASFHIPQVQVRDEGQYQCIIIYGVAWDYKYLTLKVKASYRKINTHILKVPETDEVELTCQATGYPLAEVSWPNVSVPANTSHSRTPEGLYQVTSVLRLKPPPGRNFSCVFWNTHVRELTLASIDLQSQMEPRTHPTWLLHIFIPFCIIAFIFIATVIALRKQLCQKLYSSKDTTKRPVTTTKREVNSAI


H_PDCD1LG2(PDL2) Amino acid sequence (Q9BQ51-1)：

MIFLLLMLSLELQLHQIAALFTVTVPKELYIIEHGSNVTLECNFDTGSHVNLGAITASLQKVENDTSPHRERATLLEEQLPLGKASFHIPQVQVRDEGQYQCIIIYGVAWDYKYLTLKVKASYRKINTHILKVPETDEVELTCQATGYPLAEVSWPNVSVPANTSHSRTPEGLYQVTSVLRLKPPPGRNFSCVFWNTHVRELTLASIDLQSQMEPRTHPTWLLHIFIPFCIIAFIFIATVIALRKQLCQKLYSSKDTTKRPVTTTKREVNSAI

Related products
PD-1:PD-L1(B7-H1):PDL2
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Canine_PD-1 CHO-K1 Cell LineCanine_PD-1 HEK-293 Cell LineCynomolgus_PD1 CHO-K1 Cell Line
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H_PDCD1LG2(PDL2) CHO-K1 Cell LineH_PD-L1 HEK-293 Cell LineH_PDL1 LLC1(mouse_PDL1 KO) Cell Line
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Anti-Canine_PD1 mIgG2a Antibody(3B7-D9)Anti-Canine_PD1 mIgG2a Antibody(4F12-E6)Anti-Canine_PDL1 hIgG1 Antibody(G11-6)
Anti-Feline_PD1 mIgG2a Antibody(1D8)Anti-Feline_PD1 mIgG2a Antibody(4A7)Anti-H_CD274(PDL1) hIgG1 Antibody(Atezolizumab)
Anti-H_PDCD1(PD1) hIgG1 Antibody(Budigalimab)Anti-H_PDCD1LG2 mIgG1 Antibody(3G2)Anti-Mouse PD-L1 mIgG1 Antibody(10F.9G2)
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Anti-PD-L2 hIgG1 Antibody(Hz25G4-1.1)

Biotinylated Cynomolgus PDL1 Protein; His-Avi TagBiotinylated Human PD1 Protein; His-Avi TagBiotinylated Human PDL1 Protein; His-Avi Tag
Canine PD1 Protein; hFc TagCanine PDL1 Protein; hFc TagCanine PDL1 Protein; His Tag
Cynomolgus PDL1 Protein; His TagHuman PD1 Protein; His TagHuman PDL1 Protein; His Tag
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