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Current position:Product Center > Cell lines > Immune checkpoints > PD1-PDL1 > H_PD-1 Reporter Jurkat Cell Line
H_PD-1 Reporter Jurkat Cell Line
Product Info

Cat. No:GM-C07928

Product:H_PD-1 Reporter Jurkat Cell Line


PD1-PDL1.png

Materials required

Cell Growth Medium:1640+10% FBS+0.75ug/ml Puromycin+3.5ug/ml Blasticidin+1% P.S

Cell Freezing Medium:FBS+10% DMSO

Assay Buffer:1640+1% FBS

Description


PD-1 is an immune-inhibitory receptor expressed on activated T and B cells, playing a crucial role in regulating immune responses to tumor antigens and self-antigens. The interaction of PD-1 on adjacent cells with its ligands PD-L1 or PD-L2 inhibits the transmission of TCR signaling pathways, as well as TCR-mediated cell proliferation, transcription activation, and cytokine production. Therapeutic antibodies and Fc fusion proteins that block the PD-1/PD-L1 interaction have shown promising prospects in clinical trials for treating various cancers. Current methods for measuring the activity of anti-PD-1 or anti-PD-L1 biotherapeutics rely on primary human T cells and functional endpoints such as cell proliferation, cell surface marker expression, interferon-gamma (IFNγ), and interleukin-2 (IL-2) production. These assays are challenging to establish in a quality-controlled drug development environment due to their dependence on donor primary cells, complex experimental protocols, and inadequate assay reagents. 


Genomeditech H_PD-1 Reporter Jurkat Cell Line is a reporter gene cell line that stably expresses the H_PD-1 gene and Luciferase reporter gene. When co-cultured with the corresponding aAPC(OKT3) PDL1 CHO-K1 Cell Line (Genomeditech/GM-C05269), which stably expresses human PD-L1 and a cell surface protein that activates homologous TCR in an antigen-independent manner, the interaction between PD-1 and PD-L1 inhibits TCR signaling transduction and transcription factor-mediated luciferase expression. Upon adding antibodies blocking PD-1/PD-L1, this inhibition is relieved, leading to the expression of luciferase mediated by the TCR signaling transduction and transcription factor, useful for assessing the efficacy and stability of antibodies and other biotherapeutics blocking the PD-1/PD-L1 interaction.


Data
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Anti-PD-L2 hIgG1 Antibody(Hz25G4-1.1)

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Canine PD1 Protein; hFc TagCanine PDL1 Protein; hFc TagCanine PDL1 Protein; His Tag
Cynomolgus PDL1 Protein; His TagHuman PD1 Protein; His TagHuman PDL1 Protein; His Tag
Current Position:Product Center > Cell lines > Immune checkpoints > PD1-PDL1 > H_PD-1 Reporter Jurkat Cell Line
classify
H_PD-1 Reporter Jurkat Cell Line
Product Info

Cat. No:GM-C07928

Product:H_PD-1 Reporter Jurkat Cell Line


PD1-PDL1.png

Materials required

Cell Growth Medium:1640+10% FBS+0.75ug/ml Puromycin+3.5ug/ml Blasticidin+1% P.S

Cell Freezing Medium:FBS+10% DMSO

Assay Buffer:1640+1% FBS

Description


PD-1 is an immune-inhibitory receptor expressed on activated T and B cells, playing a crucial role in regulating immune responses to tumor antigens and self-antigens. The interaction of PD-1 on adjacent cells with its ligands PD-L1 or PD-L2 inhibits the transmission of TCR signaling pathways, as well as TCR-mediated cell proliferation, transcription activation, and cytokine production. Therapeutic antibodies and Fc fusion proteins that block the PD-1/PD-L1 interaction have shown promising prospects in clinical trials for treating various cancers. Current methods for measuring the activity of anti-PD-1 or anti-PD-L1 biotherapeutics rely on primary human T cells and functional endpoints such as cell proliferation, cell surface marker expression, interferon-gamma (IFNγ), and interleukin-2 (IL-2) production. These assays are challenging to establish in a quality-controlled drug development environment due to their dependence on donor primary cells, complex experimental protocols, and inadequate assay reagents. 


Genomeditech H_PD-1 Reporter Jurkat Cell Line is a reporter gene cell line that stably expresses the H_PD-1 gene and Luciferase reporter gene. When co-cultured with the corresponding aAPC(OKT3) PDL1 CHO-K1 Cell Line (Genomeditech/GM-C05269), which stably expresses human PD-L1 and a cell surface protein that activates homologous TCR in an antigen-independent manner, the interaction between PD-1 and PD-L1 inhibits TCR signaling transduction and transcription factor-mediated luciferase expression. Upon adding antibodies blocking PD-1/PD-L1, this inhibition is relieved, leading to the expression of luciferase mediated by the TCR signaling transduction and transcription factor, useful for assessing the efficacy and stability of antibodies and other biotherapeutics blocking the PD-1/PD-L1 interaction.


Data
Related Products
PD-1:PD-L1(B7-H1):PDL2
Mouse_PDL1 KO LLC1 Cell LineMouse_PDL1 KO MC38 Cell LineaAPC(OKT3) PDL1 CHO-K1 Cell Line
H_PDCD1LG2(PDL2) aAPC CHO-K1 Cell LineMouse PDL1 aAPC CHO-K1 Cell LineMouse_PD-1 Reporter Jurkat Cell Line
Canine_PD-1 CHO-K1 Cell LineCanine_PD-1 HEK-293 Cell LineCynomolgus_PD1 CHO-K1 Cell Line
H_CD274(PD-L1) CHO-K1 Cell LineH_CD274(PD-L1) MC38 Cell LineH_PDCD1(PD-1) CHO-K1 Cell Line
H_PDCD1LG2(PDL2) CHO-K1 Cell LineH_PD-L1 HEK-293 Cell LineH_PDL1 LLC1(mouse_PDL1 KO) Cell Line
H_PDL1 MC38(mouse PDL1 KO) Cell LineH_PD-L1 Raji Cell LineM_PDCD1(PD-1) CHO-K1 Cell Line
Anti-Canine_PD1 mIgG2a Antibody(3B7-D9)Anti-Canine_PD1 mIgG2a Antibody(4F12-E6)Anti-Canine_PDL1 hIgG1 Antibody(G11-6)
Anti-Feline_PD1 mIgG2a Antibody(1D8)Anti-Feline_PD1 mIgG2a Antibody(4A7)Anti-H_CD274(PDL1) hIgG1 Antibody(Atezolizumab)
Anti-H_PDCD1(PD1) hIgG1 Antibody(Budigalimab)Anti-H_PDCD1LG2 mIgG1 Antibody(3G2)Anti-Mouse PD-L1 mIgG1 Antibody(10F.9G2)
Anti-Mouse_PD1 mIgG1 AntibodyAnti-PD1 hIgG4 Antibody(Pembrolizumab)Anti-PD1 hIgG4 Reference Antibody (Nivbio)
Anti-PD1 hIgG4 Reference Antibody (Pembio)Anti-PD1 hIgG4 Reference Antibody (Sintbio)Anti-PD-1 hIgG4 Reference Antibody (Torbio)
Anti-PD1 hIgG4 Reference Antibody(Cambio)Anti-PD-1 hIgG4 Reference Antibody(Tislbio)Anti-PDL1 hIgG4 Reference Antibody(Adebio)
Anti-PD-L2 hIgG1 Antibody(Hz25G4-1.1)

Biotinylated Cynomolgus PDL1 Protein; His-Avi TagBiotinylated Human PD1 Protein; His-Avi TagBiotinylated Human PDL1 Protein; His-Avi Tag
Canine PD1 Protein; hFc TagCanine PDL1 Protein; hFc TagCanine PDL1 Protein; His Tag
Cynomolgus PDL1 Protein; His TagHuman PD1 Protein; His TagHuman PDL1 Protein; His Tag
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