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Current position:Product Center > Cell lines > Immune checkpoints > PD1-PDL1 > Mouse_PD-1 Reporter Jurkat Cell Line
Mouse_PD-1 Reporter Jurkat Cell Line
Product Info

Cat. No:GM-C25661

Product:Mouse_PD-1 Reporter Jurkat Cell Line


H_PD-1 PDL1 NFAT Reporter Blockade Assay信号通路.png

Materials required

Cell Growth Medium:RPMI 1640+10% FBS+1% P.S+0.75 μg/mL Puromycin+3.5 μg/mL Blasticidin

Cell Freezing Medium:90% FBS+10% DMSO

Assay Buffer:RPMI 1640+1% FBS+1% P.S

Description

PD-1 is an immunosuppressive receptor expressed on activated T cells and B cells, playing a critical role in regulating immune responses to tumor antibodies and self-antigens. Interaction between PD-1 on neighboring cells and its ligands PD-L1 or PD-L2 inhibits TCR signaling pathway transduction, as well as TCR-mediated cell proliferation, transcription activation, and cytokine production. Therapeutic antibodies and Fc fusion proteins blocking PD-1/PD-L1 interactions have shown promising application prospects in clinical trials for various cancers. Current methods for evaluating the activity of anti-PD-1 or anti-PD-L1 biological products rely on measuring primary human T cell and functional endpoints such as cell proliferation, cell surface marker expression, interferon-gamma (IFNγ), and interleukin-2 (IL-2) production. Due to reliance on donor primary cells, complex experimental protocols, and substandard reagents, these assays are both labor-intensive and prone to variability. Therefore, these assay methods are challenging to establish in a quality-controlled drug development environment.


The Genomeditech Mouse_PD-1 Reporter Jurkat Cell Line is a reporter gene cell line stably expressing the Mouse_PD-1 gene and Luciferase reporter gene. Paired with the Mouse PDL1 aAPC CHO-K1 Cell Line (Genomeditech/GM-C25791), which stably expresses mouse PD-L1 and a cell surface protein that activates endogenous TCR in an antigen-independent manner. When both cell lines are co-cultured, the interaction between PD-1/PD-L1 inhibits TCR signaling pathway transduction and transcription factor-mediated luciferase expression. Addition of a blocking antibody against PD-1/PD-L1 reverses this inhibition, leading to TCR signaling pathway transduction and transcription factor-mediated luciferase expression, which can be used to assess the efficacy and stability of blocking antibodies against PD-1/PD-L1 interactions and other biological products.


Data
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Anti-PD-L2 hIgG1 Antibody(Hz25G4-1.1)

Biotinylated Cynomolgus PDL1 Protein; His-Avi TagBiotinylated Human PD1 Protein; His-Avi TagBiotinylated Human PDL1 Protein; His-Avi Tag
Canine PD1 Protein; hFc TagCanine PDL1 Protein; hFc TagCanine PDL1 Protein; His Tag
Cynomolgus PDL1 Protein; His TagHuman PD1 Protein; His TagHuman PDL1 Protein; His Tag
Current Position:Product Center > Cell lines > Immune checkpoints > PD1-PDL1 > Mouse_PD-1 Reporter Jurkat Cell Line
classify
Mouse_PD-1 Reporter Jurkat Cell Line
Product Info

Cat. No:GM-C25661

Product:Mouse_PD-1 Reporter Jurkat Cell Line


H_PD-1 PDL1 NFAT Reporter Blockade Assay信号通路.png

Materials required

Cell Growth Medium:RPMI 1640+10% FBS+1% P.S+0.75 μg/mL Puromycin+3.5 μg/mL Blasticidin

Cell Freezing Medium:90% FBS+10% DMSO

Assay Buffer:RPMI 1640+1% FBS+1% P.S

Description

PD-1 is an immunosuppressive receptor expressed on activated T cells and B cells, playing a critical role in regulating immune responses to tumor antibodies and self-antigens. Interaction between PD-1 on neighboring cells and its ligands PD-L1 or PD-L2 inhibits TCR signaling pathway transduction, as well as TCR-mediated cell proliferation, transcription activation, and cytokine production. Therapeutic antibodies and Fc fusion proteins blocking PD-1/PD-L1 interactions have shown promising application prospects in clinical trials for various cancers. Current methods for evaluating the activity of anti-PD-1 or anti-PD-L1 biological products rely on measuring primary human T cell and functional endpoints such as cell proliferation, cell surface marker expression, interferon-gamma (IFNγ), and interleukin-2 (IL-2) production. Due to reliance on donor primary cells, complex experimental protocols, and substandard reagents, these assays are both labor-intensive and prone to variability. Therefore, these assay methods are challenging to establish in a quality-controlled drug development environment.


The Genomeditech Mouse_PD-1 Reporter Jurkat Cell Line is a reporter gene cell line stably expressing the Mouse_PD-1 gene and Luciferase reporter gene. Paired with the Mouse PDL1 aAPC CHO-K1 Cell Line (Genomeditech/GM-C25791), which stably expresses mouse PD-L1 and a cell surface protein that activates endogenous TCR in an antigen-independent manner. When both cell lines are co-cultured, the interaction between PD-1/PD-L1 inhibits TCR signaling pathway transduction and transcription factor-mediated luciferase expression. Addition of a blocking antibody against PD-1/PD-L1 reverses this inhibition, leading to TCR signaling pathway transduction and transcription factor-mediated luciferase expression, which can be used to assess the efficacy and stability of blocking antibodies against PD-1/PD-L1 interactions and other biological products.


Data
Related Products
PD-1:PD-L1(B7-H1):PDL2
Mouse_PDL1 KO LLC1 Cell LineMouse_PDL1 KO MC38 Cell LineaAPC(OKT3) PDL1 CHO-K1 Cell Line
H_PD-1 Reporter Jurkat Cell LineH_PDCD1LG2(PDL2) aAPC CHO-K1 Cell LineMouse PDL1 aAPC CHO-K1 Cell Line
Canine_PD-1 CHO-K1 Cell LineCanine_PD-1 HEK-293 Cell LineCynomolgus_PD1 CHO-K1 Cell Line
H_CD274(PD-L1) CHO-K1 Cell LineH_CD274(PD-L1) MC38 Cell LineH_PDCD1(PD-1) CHO-K1 Cell Line
H_PDCD1LG2(PDL2) CHO-K1 Cell LineH_PD-L1 HEK-293 Cell LineH_PDL1 LLC1(mouse_PDL1 KO) Cell Line
H_PDL1 MC38(mouse PDL1 KO) Cell LineH_PD-L1 Raji Cell LineM_PDCD1(PD-1) CHO-K1 Cell Line
Anti-Canine_PD1 mIgG2a Antibody(3B7-D9)Anti-Canine_PD1 mIgG2a Antibody(4F12-E6)Anti-Canine_PDL1 hIgG1 Antibody(G11-6)
Anti-Feline_PD1 mIgG2a Antibody(1D8)Anti-Feline_PD1 mIgG2a Antibody(4A7)Anti-H_CD274(PDL1) hIgG1 Antibody(Atezolizumab)
Anti-H_PDCD1(PD1) hIgG1 Antibody(Budigalimab)Anti-H_PDCD1LG2 mIgG1 Antibody(3G2)Anti-Mouse PD-L1 mIgG1 Antibody(10F.9G2)
Anti-Mouse_PD1 mIgG1 AntibodyAnti-PD1 hIgG4 Antibody(Pembrolizumab)Anti-PD1 hIgG4 Reference Antibody (Nivbio)
Anti-PD1 hIgG4 Reference Antibody (Pembio)Anti-PD1 hIgG4 Reference Antibody (Sintbio)Anti-PD-1 hIgG4 Reference Antibody (Torbio)
Anti-PD1 hIgG4 Reference Antibody(Cambio)Anti-PD-1 hIgG4 Reference Antibody(Tislbio)Anti-PDL1 hIgG4 Reference Antibody(Adebio)
Anti-PD-L2 hIgG1 Antibody(Hz25G4-1.1)

Biotinylated Cynomolgus PDL1 Protein; His-Avi TagBiotinylated Human PD1 Protein; His-Avi TagBiotinylated Human PDL1 Protein; His-Avi Tag
Canine PD1 Protein; hFc TagCanine PDL1 Protein; hFc TagCanine PDL1 Protein; His Tag
Cynomolgus PDL1 Protein; His TagHuman PD1 Protein; His TagHuman PDL1 Protein; His Tag
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