Genomeditech：GM-C24030
Product：H_GIPR Reporter HEK-293 Cell Line
Genomeditech：GM-C24030
Product：H_GIPR Reporter HEK-293 Cell Line
Cell Growth Medium：DMEM+10% FBS+1% P.S+0.75 μg/mL Puromycin+4 μg/mL Blasticidin
Cell Freezing Medium：90% FBS+10% DMSO
Assay Buffer：DMEM+1% FBS+1% P.S
Gastric inhibitory polypeptide receptor (GIPR) is a protein encoded by the GIPR gene in the human body, activated by gastric inhibitory polypeptide (GIP), and is a member of the G protein-coupled receptor family. GIPRs are mainly found in the β cells of the pancreas. Activation of GIPR by gastric inhibitory polypeptide leads to the binding with heterotrimeric Gs (αβγ), inducing adenylyl cyclase activation, increasing cAMP levels in the cytoplasm, activating PKA, phosphorylating transcription factors, and resulting in their translocation to the cell nucleus.
The H_GIPR Reporter HEK-293 Cell Line from Genomeditech is a luciferase reporter cell line constructed based on the GIPR signaling pathway. When gastric inhibitory polypeptide binds with its receptor GIPR, it phosphorylates transcription factors, leading to activation of luciferase expression. The luciferase reading represents the activation effect of the signaling pathway, thus can be used for in vitro evaluation of GIPR-related drug effects.
Genomeditech：GM-C24030
Product：H_GIPR Reporter HEK-293 Cell Line
Cell Growth Medium：DMEM+10% FBS+1% P.S+0.75 μg/mL Puromycin+4 μg/mL Blasticidin
Cell Freezing Medium：90% FBS+10% DMSO
Assay Buffer：DMEM+1% FBS+1% P.S
Gastric inhibitory polypeptide receptor (GIPR) is a protein encoded by the GIPR gene in the human body, activated by gastric inhibitory polypeptide (GIP), and is a member of the G protein-coupled receptor family. GIPRs are mainly found in the β cells of the pancreas. Activation of GIPR by gastric inhibitory polypeptide leads to the binding with heterotrimeric Gs (αβγ), inducing adenylyl cyclase activation, increasing cAMP levels in the cytoplasm, activating PKA, phosphorylating transcription factors, and resulting in their translocation to the cell nucleus.
The H_GIPR Reporter HEK-293 Cell Line from Genomeditech is a luciferase reporter cell line constructed based on the GIPR signaling pathway. When gastric inhibitory polypeptide binds with its receptor GIPR, it phosphorylates transcription factors, leading to activation of luciferase expression. The luciferase reading represents the activation effect of the signaling pathway, thus can be used for in vitro evaluation of GIPR-related drug effects.
