Current position:Product Center > Antibodies > Loading Controls Antibodies & Tag Antibodies > Anti-GAPDH mIgG2a Antibody
Anti-GAPDH mIgG2a Antibody
Product info

Cat.No:GM-77904AB

Product:Anti-GAPDH mIgG2a Antibody



Cat. No. & Size

GM-77904AB-10        

GM-77904AB-100         

GM-77904AB-1000       


Description️

Source/Isotype           Monoclonal Mouse IgG2a

Species Reactivity       Human, Mouse, Canine, Rat, Pig

Conjugate                   Unconnjugated

Other Names              G3PD, GAPD, GAPDH, OK/SW cl.12

Application                 Western blotting: 1:1000-1:5000; WB-Quality tested

Background                GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. One of the GAPDH moonlighting function is its role in adhesion and binding to other partners. GAPDH can itself activate transcription. GAPDH acts as a reversible metabolic switch under oxidative stress. GAPDH also appears to be involved in the vesicle transport from the endoplasmic reticulum (ER) to the Golgi apparatus which is part of shipping route for secreted proteins. Because the GAPDH gene is often stably and constitutively expressed at high levels in most tissues and cells, it is considered a housekeeping gene. For this reason, GAPDH is commonly used by biological researchers as a loading control for western blot and as a control for qPCR.

Formulation                20mM Histine, 50% glycerinum, 0.05% Bacteriostatic preservative, pH 6.0.


Data

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Current position:Product Center > -- > Anti-GAPDH mIgG2a Antibody
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Anti-GAPDH mIgG2a Antibody
Product info

Cat.No:GM-77904AB

Product:Anti-GAPDH mIgG2a Antibody



Cat. No. & Size

GM-77904AB-10        

GM-77904AB-100         

GM-77904AB-1000       


Description️

Source/Isotype           Monoclonal Mouse IgG2a

Species Reactivity       Human, Mouse, Canine, Rat, Pig

Conjugate                   Unconnjugated

Other Names              G3PD, GAPD, GAPDH, OK/SW cl.12

Application                 Western blotting: 1:1000-1:5000; WB-Quality tested

Background                GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. One of the GAPDH moonlighting function is its role in adhesion and binding to other partners. GAPDH can itself activate transcription. GAPDH acts as a reversible metabolic switch under oxidative stress. GAPDH also appears to be involved in the vesicle transport from the endoplasmic reticulum (ER) to the Golgi apparatus which is part of shipping route for secreted proteins. Because the GAPDH gene is often stably and constitutively expressed at high levels in most tissues and cells, it is considered a housekeeping gene. For this reason, GAPDH is commonly used by biological researchers as a loading control for western blot and as a control for qPCR.

Formulation                20mM Histine, 50% glycerinum, 0.05% Bacteriostatic preservative, pH 6.0.


Data

image.png

Relevant data
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