| Species Reactivity | Human; Cynomolgus |
| Clone | PY159 |
| Source/Isotype | Human IgG1, Kappa |
| Application | Bioactivity-ELISA |
| Target | Detects TREM1 |
| Gene | TREM1 |
| Other Names | CD354, TREM-1 |
| Gene ID | 54210 (Human); Cynomolgus (102129811) |
| Background | TREM-1 is a cell-surface Ig superfamily receptor mainly on neutrophils and monocytes/macrophages. It amplifies inflammatory responses by boosting proinflammatory cytokines/chemokines after activation. It acts as an amplifier of signals from pattern recognition receptors (e.g., TLRs) and contributes to host defense as well as inflammatory/septic pathogenesis. TREM-1 is modulated by the adaptor DAP12, which contains an ITAM for downstream signaling. Ligand engagement crosslinks TREM-1 with DAP12, phosphorylating the ITAM via Src kinases. This recruits Syk, triggering a cascade that activates NF-κB and MAPKs (ERK, p38, JNK). This leads to transcription of TNF-α, IL-1β, IL-6, and chemokines. TREM-1 signaling can amplify TLR signals and is shaped by cross-talk with other receptors, as well as regulation by soluble TREM-1 and inhibitory receptors to limit excessive inflammation. |
| Storage | Store at 2-8℃ short term (1-2 weeks).Store at ≤ -20℃ long term. Avoid repeated freeze-thaw. |
| Formulation | Supplied as a 0.2 μm filtered solution of PBS, pH7.2-7.4. |
| Endotoxin | < 1 EU/mg, determined by LAL gel clotting assay |
| Species Reactivity | Human; Cynomolgus |
| Clone | PY159 |
| Source/Isotype | Human IgG1, Kappa |
| Application | Bioactivity-ELISA |
| Target | Detects TREM1 |
| Gene | TREM1 |
| Other Names | CD354, TREM-1 |
| Gene ID | 54210 (Human); Cynomolgus (102129811) |
| Background | TREM-1 is a cell-surface Ig superfamily receptor mainly on neutrophils and monocytes/macrophages. It amplifies inflammatory responses by boosting proinflammatory cytokines/chemokines after activation. It acts as an amplifier of signals from pattern recognition receptors (e.g., TLRs) and contributes to host defense as well as inflammatory/septic pathogenesis. TREM-1 is modulated by the adaptor DAP12, which contains an ITAM for downstream signaling. Ligand engagement crosslinks TREM-1 with DAP12, phosphorylating the ITAM via Src kinases. This recruits Syk, triggering a cascade that activates NF-κB and MAPKs (ERK, p38, JNK). This leads to transcription of TNF-α, IL-1β, IL-6, and chemokines. TREM-1 signaling can amplify TLR signals and is shaped by cross-talk with other receptors, as well as regulation by soluble TREM-1 and inhibitory receptors to limit excessive inflammation. |
| Storage | Store at 2-8℃ short term (1-2 weeks).Store at ≤ -20℃ long term. Avoid repeated freeze-thaw. |
| Formulation | Supplied as a 0.2 μm filtered solution of PBS, pH7.2-7.4. |
| Endotoxin | < 1 EU/mg, determined by LAL gel clotting assay |
